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KMID : 1024520080170121325
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Journal of the Environmental Sciences
2008 Volume.17 No. 12 p.1325 ~ p.1330
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Development of Multi-Residue Methods for Carbamate Pesticides by the Enzyme Inhibition Test
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Kim Jung-Ho
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Abstract
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This study was carried out with the detection for multiresidue of the carbamate pesticide such as carbaryl and cabofuran by enzyme-inhibition method. The check time for determination of acetylcholinesterase(AChE) activity was selected at 60 sec. The AChE activity in chicken brain determined by the Ellman’s method was mol/min/g protein. for AChE by carbamate pesticide with wet kit was 0.169mg/L of carbaryl and 0.089mg/L of cabofuran, respectively. The incubation time for enzyme kit with substrate kit was 30min for determination of AChE activity. Enzyme kit with substrate kit was stable at for 5 days. Limit detection concentration of carbaryl with dry kit for AChE was 0.05mg/L. The dry kit such as wet kit applied Enzyme-Inhibition(EI) method with AChE was confirmed the multi residue method to detect the carbamate pesticides.
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KEYWORD
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Enzyme inhibition, Acetylcholinesterase, Multiresidue method, Carbamate pesticides
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